Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MAKLIVLGGLIYGG-------YWLYKYMLAHPNIDKGKVIKTV--GVIIVGLIALTKKNNT---NSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID----------------------------------------------------------------------------------------------------------------- 4D10 Chain:C ((3-403)) SALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQF

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 308 -5522 -17.93 -56.35 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.57 3D Compatibility (PKB) : -17.93 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D10-query.scw PDB file : Tito_Scwrl_4D10.pdb: