Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELP-E-EFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPG-E----ITDNNGI-LTFNFSDPEGNYFVIGKAESSD 4HC5 Chain:B ((8-132)) ---------------SLMIAYVHSATIIVSDQEKALDFYVNTLGFEKVFDNQLDPNMRFVTVVPPGA-QTQVALGLPSWYED--GRKPGGYTGISLITRDIDEAYKTLTERGVTFTKPPEMMPWGQRATWFSDPDGNQFFLVE-----

General information: TITO was launched using: RESULT: Template: 4HC5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 399 -16744 -41.96 -143.11 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -41.96 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_4HC5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HC5-query.scw PDB file : Tito_Scwrl_4HC5.pdb: