Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MIYKGTLLYKPEECSHCLCVVPSRIIRWG--TTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR-VKETTVRLKGNSHSQRGLGKN 5E4B Chain:A ((34-209)) EQFQLRGVLW--------------GKAYSWKITGTTIDKVWSIVGD---YVRVDN-----------------------WVSSVVKSSHVVSGEANQT----------------GCVRRFVC--YPASEGESETVDYSELIHMNAAAHQYMYMIVGG------NI---TGFSLMKNYVSNISLSSLPEEDGGGVIFYWSFTAEPASNLTEQKCIEIVFPLYTTALKDLCTHLSIPESSVTLLDD----------

General information: TITO was launched using: RESULT: Template: 5E4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 783 -11076 -14.14 -64.77 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -14.14 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_5E4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E4B-query.scw PDB file : Tito_Scwrl_5E4B.pdb: