Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFHSTPNKGGTFDMTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQLLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFYTSEEKAKAHLDAGVKRVVISAPA-GAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPMAYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAIGLVIPELQGKLQGHAQRVPVVDGSLTELVSVL-KTKVTADQVNEAIKKHTVDNPSFGYDDRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFANL 3VAZ Chain:A ((10-342)) ---------------AVKVAINGFGRIGRLAFRRIQEV-EGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPW-KDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVAKVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIGKVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNAS--NESFGYTEDEIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL

General information: TITO was launched using: RESULT: Template: 3VAZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2008 -68816 -34.27 -207.90 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -34.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_3VAZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VAZ-query.scw PDB file : Tito_Scwrl_3VAZ.pdb: