Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAVMISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMAGLGAHSVIVTNACGGVNESFVPGDLMLITDHIN---FTGENPLIGPNEDAMGPRFPDMSQAYTPDYQATAKAAAEKLG--LDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEVIVAAHSGLKVLGISCITNLAAGMQANL---SHEEVVETTQRVKQSFKALVKETLTLL 2Q7O Chain:E ((8-275)) -----EDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFV-------

General information: TITO was launched using: RESULT: Template: 2Q7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1356 -136108 -100.37 -523.49 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -100.37 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_2Q7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q7O-query.scw PDB file : Tito_Scwrl_2Q7O.pdb: