Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MMAELKKMQDYE-----YDLPELVDQPEQQMPDSSSNQEIILPQSPAKRLKQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQEKNELSRTERIKQIA--------EEKGLSINDDNLRKVK------------------------------------- 5H9D Chain:C ((3-190)) TTVSKLERQIEERLKGVSEYESININHRLGKLLDSYD--IPDVAKVACLTIDTS-MRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIVEINELKSSLHH--QALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDYYPSYFKKYNQSEVKHYLHDIQKSYLKSRGN

General information: TITO was launched using: RESULT: Template: 5H9D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 411 27671 67.33 214.50 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 67.33 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_5H9D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H9D-query.scw PDB file : Tito_Scwrl_5H9D.pdb: