Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSITLLQPDDWHAHLRDG-------LALKR-TVPDLAKQFARAICMPNLVPPVKTVEEALAYRERILAHVPEGNNFDPRMVLYFTDH--TSPDEVRKIKE-SEHVNAIKLYPAGATTNSNNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHNHVD---------------------IFDREKRFLDEVLSPLLKQFPKLKVVLEHITTSDAAHFVLEQD---RNVAATITPQHLLFNRNDMLVGG--IKPYFYCLPILKRQ-THQTTLLEVATSGNPKFFLGTDSAPHAQNAKEN-----ACGCAGCYSAPNAIELYAQAF-D-QVGKLERLEGFASHFGADFYGLPRNTSTITLVKEDNLVPESFDYLDNQKIIPLHAGKTLQWRKV 1KCX Chain:B ((62-406)) -GRMVIPGGIDVNTYLQKPSQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKS--CCDYSLHVD--ITSWYDGVREELEVLVQDKG-VNSFQVYMAYKDL---Y-QMSDSQLYEAFTFLKGLGAVILVHAENGDL-IAQEQKRILEMGITGPEGHALSRPEELEAEAVFRA-IAIA-GRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDL-QVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSD------------------------------

General information: TITO was launched using: RESULT: Template: 1KCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1704 53163 31.20 177.21 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 31.20 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_1KCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KCX-query.scw PDB file : Tito_Scwrl_1KCX.pdb: