Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIAKLKIAIIGLGYVGLPLAVEFGKK-VPVVGFDIYQKRIDELKNGQDHTLEVSPEE-LKQAV---HLKYTAH-LDDLQDSNFFIVTVPTPIDDFKQPDLTPLIKASTSIGQVLK---KGDVVVYESTVYPGATEEVCIPVLEKISGLKFNQDFFAGYSPERINPGDKQHRVTNILKITSGS-TPEVADYIDEVYNLIIEAGTHKAPNIKVAEAAKVIENTQRDVNIALINELALIFNKLDIDTEDVLKAAGTK--WNFLPFRPGL-VGGHCIGVDPYYLTHKAQSIGLHPEIILAARRLNDRMGEHVATQLIKEMVKQRIQVVGARILVMGLSFKENCPDIRNTKIVDFIKALKEYDLDLDIYDPWVDDTEVQH---EYGLAPIKELQQGLYDAIVIAVAHNQFKSMSVEEFHALGKEKHVLYDLKYVLDKESSNIRL 3VTF Chain:A ((17-436)) RGSHMASLSVLGLGYVGVVHAVGFALLGHRVVGYDVNPSIVERLRAGRPHIYEPGLEEALGRALSSGRLSFAESAEEAVAATDATFIAVGTPPAPDGSADLRYVEAAARAVGRGIRAKGRWHLVVVKSTVPPGTTEGLVARAVAE--EAG-GVKFSVASNPEFLREGSALEDFFKPDRIVIGAGDERAASFLLDVYKAVDAP-KLVM-KPREAELVKYASNVFLALKISFANEVGLLAKRLGVDTYRVFEAVGLDKRIGRHYFGAGLGFGGSCFPKDTLAFIRFGESLGLEMAISKAVLRVNEYMPRYAVQLLEERLG----GLRGRHVGVLGLAFKPNTDDVRESRGVEVARLLLERGARVYVHDPMAMEKARAVLGDSVTYVEDPQALLDQVEGVIIATAWPQYEGLD--Y------RGKVVVDGRYVKKAREAK---

General information: TITO was launched using: RESULT: Template: 3VTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2242 14245 6.35 35.26 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 6.35 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_3VTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VTF-query.scw PDB file : Tito_Scwrl_3VTF.pdb: