Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------MKKLAIASALLSALAVSGAANAYQAEVGGSYNYLDPDNGSSVSKFGVDGTYYFNPVQTRNAPLAEAAFLNRASNVNAHVNYGDNSGTKDTQ-YGVGVEYFVPNSD----FYLSGDVGRNEREIDNTNIDSKVTTYAAEVGYLPAPGLLLALGVKGYDEKD-GKDGADPTVRAKYVTQV-GQHDVNLEAYGAFGD-LDEYKVRGDYYIDKTLSLGVDYYNNDLTDKDEFGINAKKFLNQQVSVEGRVGFGDNDNTYGVRAAYRF-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 5H8Y Chain:D ((11-576)) KRSKVEIIKEKSNFLRYPLNEELVSAPNINESAVQLIKFHGSYQQTDRDVQKNYSFMLRTKNPCGKVPNQLYLAMDTLADEFGIGTLRLTTRQTFQLHGVLKKNLKTVLSTVIKNMGSTLGACGDLNRNVLAPAAPYVKKDILFAQQTAENIAALLTP---QSGAYYDLWVDEEPPEVTKARNDNGTNFPD---SPEPIYGTQYLPRKFKVAVTAA-GDNSVDILTNDIGVVVVSDDAGEPIGFNIYVGGGMGRTHR------VETTFPRLADPLGYVPKEDILYAIKAIVVTQRENGRRDDRKYSRMKYMIDRWGIDRFRAEVEKYYGKKFESFRPLPEWQFNSYLG-----WQEQGDGKLFYGVHVDN---------GRVGGQAKKTLREIIEKYNLDVSITPNQNLILCGIDQAWREPITTALAQAGLLEPKDVDPLNLTAMACPALPLCPLAQTEAERGILPILKRIRAVFNKVGIKDSESVVVRITGCPNGCARPYMAELGFVGDGPKSYQIWLGGTPNQSTLAESFMDKVKLDDIEKVLEPLFTYWNGTRQEGESFGSFTNRTGFDKLKEVVNKW

General information: TITO was launched using: RESULT: Template: 5H8Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1141 65695 57.58 288.13 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain D : 0.59 3D Compatibility (PKB) : 57.58 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.110

(partial model without unconserved sides chains): PDB file : Tito_5H8Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H8Y-query.scw PDB file : Tito_Scwrl_5H8Y.pdb: