Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQGVR-GNGVIRKLAAPFK--------ILKATFSAMRYMKQLKVDAVAGFGGYVAGPGGLAARLLGIPVLIHEQNAVA---------------GF---TN---AQLSRV-------AKVVCEAFPNT---FPAS----EKVVTTGNPVRREITDILSPKWRYDEREQAGKPLNILIVGGSLG-------AKALNERLPPALKQLQVPLNIFHQCGQQQVEATQALYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLL-TEMAVKARQHAQPNATQHVVGLIQKM 4LEI Chain:A ((15-437)) -------SHMRVLFTPLPASSHFFNLVPLAWALRAAGHEVRVAICPNMV--SMVTGAGLTAVPVGDELDLISL----------DAVEQLHLVDDRSLDDLMGFAEKWQPDLVVWDAMV--CSGPVVARALGARHVRMLVALDVSGWLRSGFLEYQESKPPEQRVDPLGTWLGAKLAKFGATFDEEIVTGQATIDPIPSWMRLPVDLDYISMRFVPYNGP---A-VLPEWLRERPTKPRVCITRGLTKRRLSRVEQAMV-ERLLRGAARL--DVEVIATLSDDEV--------ELPSN--VRVHEYV-PLNELLESCSVIIHHGSTTTQETATVNGVPQLILP------WDESRRAELLADRGAGLVLDPATFTEDDVRGQLARLLDEPSFAANAALIRREIEESPSPHDIVPRLE--

General information: TITO was launched using: RESULT: Template: 4LEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1659 -100857 -60.79 -317.16 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -60.79 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_4LEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LEI-query.scw PDB file : Tito_Scwrl_4LEI.pdb: