Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFLQRSNQHSNNNRLKRMKSHLRVHYFQHIAGEGFGSCYSFLKAHHATITATEFFALPVDLPLEIEALPYIEDVDLLLIMGGTMSVNDEANYPWLKIEKRWIRRYLAAGKPAIGLCLGGQLIANALGAAVSRNRYQELGWSTVQRVPNLPKESFL--LPEKINVMQWHSETF-EIPKGAIHLAENSVCRNQMYQIG-SNVLGFQFHPEMTPK-----VLNLLLENEQELSIFKGEYVQSLDELHHCDIQKFEQGNQLLNRAIEFVVNQ 5TW7 Chain:C ((9-227)) --------------------MTQDKILILDFGS-QVTRLIARRVREAH--VYCELH--SFD---MPLDEI-KAFNPKGIILSGGPNSVYES-DY-------QADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQREFGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMENTEKQFYGIQFHPEVTHTKQGRALLNRFVLD-------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TW7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1038 -94134 -90.69 -520.08 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -90.69 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_5TW7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TW7-query.scw PDB file : Tito_Scwrl_5TW7.pdb: