Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGSMNAIPQTFEFWQDRRMPYVETRRSCFGRTCYKSHSHPTFSIGAIDEGNSVFQSSFGTAQKITAGTLVIVPAYVEHSCNPMPNQAWSYQMLHLDLAWLNQLYSEFQEQGLDLHIPQHKPLIIKDESLYETFTEMNETLFDAQKLIFEKEQALLHCLIHLLLPHFILEEIQKPQYLYKDFLNLIDVI-SSSE-GFISLEELAQRVGLSRYAIIRLFKANVGLTPHAFQINLKINQAREQLKQ-GVPLAELAVNLGFNDQSHFHKAFKAHTGVTPRQFQLAAAQ 3LSG Chain:A ((3-103)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AKELIQNIIEESYTDSQFTLSVLSEKLDLSSGYLSIMFKKNFGIPFQDYLLQKRMEKAKLLLLTTELKNYEIAEQVGFEDVNYFITKFKKYYQITPKQYRE----

General information: TITO was launched using: RESULT: Template: 3LSG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 319 5945 18.64 60.66 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 18.64 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.739

(partial model without unconserved sides chains): PDB file : Tito_3LSG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LSG-query.scw PDB file : Tito_Scwrl_3LSG.pdb: