Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFFVLSCTDNEGTLEKRLAVRPQHIE--RLQKLDDEGRLVAAGAMPKDPNDPQVGFYGSTMIVEFDTREALDEWLKEEPFLKEGIYSHVDVKPFNKAFPKG 3ZNU Chain:A ((1-94)) MLYLVRMTVNLPRNLDPREEERLKASEKARSRTLQEQGQWRYLWRTT--------GKYGNISVFDVNSHDELHEILWSLPFFPYLTIDVEPLSHHPARVGKD

General information: TITO was launched using: RESULT: Template: 3ZNU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 290 -5131 -17.69 -55.77 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -17.69 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3ZNU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZNU-query.scw PDB file : Tito_Scwrl_3ZNU.pdb: