Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKSPGFTIVELTITLVILVIMSVIAIPLYHQFMASVELKNTLRILTIHIQKAKYDAIIRHKNVVLCPSSDQLVCNTNWDNRIISFVDNNRNLQHDLNEELLASIDLNHHYGSMKLQRFGKKQNSIIFQGNSGLPIESNGSFIYCSYDQLKKREFGLSRI 1PRT Chain:D ((1-110)) --------DVPYVLVKTNMVVTSVAMKPY--------EVTPTRMLVCGIAAKLGAAASSPDAHVPFCFGKDL------------------KRPGSSPMEVMLRAVFMQQRPLRMFL---GPKQLTF-----EGKPALELIRMVECS----GKQDCP----

General information: TITO was launched using: RESULT: Template: 1PRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 478 -65856 -137.77 -598.69 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain D : 0.60 3D Compatibility (PKB) : -137.77 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_1PRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PRT-query.scw PDB file : Tito_Scwrl_1PRT.pdb: