Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLMIDNYDSFTYNIVQYFGELNQEVKVVRNDQVTL---EDIERWQPKYLVIGPGPCSPSEVGISIPAINHFA-GKIPLFGVCLGHQSIGQAFGGKIVRAKTVMHGRLSDMYHSNKGIFSNLPSPFSATRYHSLVIDQETLPDCLEVTCWTNEADGSMEEIMGVKHKTLPVEGVQFHPESILSQHGHQIFKNFLDIYA 1I7S Chain:B ((3-190)) DILLLDNVDSFTYNLVDQLRASGHQVVIYRNQIGAEVIIERLQHMEQPVLMLSPGPGTPSEAGC-MPELLQRLRGQLPIIGICLGHQAIVEAYGGQVGQAGEILHGKASAIAHDGEGMFAGMANPLPVARYHSLVGS--NIPADLTVNARFG------EMVMAVRDDRRRVCGFQFHPESILTTHGARLLEQTLAWA-

General information: TITO was launched using: RESULT: Template: 1I7S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1033 -38442 -37.21 -208.92 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -37.21 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_1I7S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I7S-query.scw PDB file : Tito_Scwrl_1I7S.pdb: