Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFERGIGFFALIFSILVIGAFIALSIYLIRLDNIIREKFEGQRWDIPAKVFARPLEIYNNAPITQANFTQELKLLGYKTSSNYDKSGTYVAQGSNMYVHTRGFDYGDSVEPEQVLELSFANDQVVEVRSTKPSSTGVARLEPLLIGGIYPQHNEDRVLIKLNSVPKPLIEALISTEDRNFYHHHGISIRGTARALVSNVTGGRRQGGSTLTQQLVKNFYLTPERTLKRKVNEALMALLIELHYSKDEILEAYLNEVNLGQNGNYSINGYGLAAQFYFGLPLRELNVAQQAYLVGLVQGPSLYNPWKNPEGAKNRRDTVLNNMRVMGYLTQAEYETEIARPLNVLSKP-SLGPAKFPDFLDIVRRQLRTEY--QESDLTNQGLRIFTTLDPIAQTQVQNAFKASVERLANSNPARLKNLQGAVLIAHPENGELIAAVGST-QD-FTGFNRALDAKRQVGSLLKPV-IYLSAIESGRYNWASQIEDAPISVPVDGGKSWTPKNYSGGGHGIVSLSEALANSYNLSAVRLGQEFGLSTFTNNLRKFGVESTI-PAYPSIFLGA--VNMSPMEVLGIYENFATGGFKYPTRAIRSVVDANGHLLDRYGLNVQETIDPSVGYIMNYGLQQVMSSGTGRAAYNSLSPALKLAGKSGTTN-------DTRDSWFAGYSGNHVAVVWLGLDDNKVTG---LTGSSGALPVWINVMKQ-LRQ-TPVNLRQPDSVQWQWIDHASGDLSAQACDGAMYIPMLAHTVPHRATPCGAPYYQVDPTYTPQSDNTIPEPEDDNTDSYIRESENQMEQDLSNNTRIISSGSYNN 3ZG9 Chain:B ((9-464)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KDEIMEMYLNRSYFGN----GEWGVENASLKYFGKSAADLNIPEAATIAGLLQAPSAYDPYQHIDKATNRRNMVLNAMVETGTISKAEGDKYKATKIVLNDQSKDPLANKYPWYVDAVINEAVNEADITQDEIMQKGYKIYTELDQNYQTSLENVYNNDGLFPSNAN--DGTLVQSGAVLMDPATGGIRALVGGRGEHVFRGFNRATQMKAQPGSTMKPLAVYTPALQSG-YDVDSMLKDEKITYK----GNYTPTNVGGVYSGEVPMYKAVANSINAPAVWLLDQIGIDKGVKSVEKFGITVPEKDRTLGLALGGMSKGASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVPKTKQIISETVSNEMTSMLLDVINTGTGQSAA---VSGHEMAGKTGSTQVPFDDTSGTKDQWFVGYTPNLVGAVWMGYDKTDKEHYLTTTSSAGVSSLAHYVMNSGLQYQ--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2392 43251 18.08 99.43 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 18.08 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_3ZG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZG9-query.scw PDB file : Tito_Scwrl_3ZG9.pdb: