Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYKLTADMPGCDKEDTTVEYSD-NTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLP-NVDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN 5DS1 Chain:B ((2-94)) ------------------------------STPADVKEHPNSYVFMVDMPGVKSGDIKVQVEDENVLLISGERKRE---KEGVKYLKMERRIGKLMRKFVLPEN-NIEAISAISQDGVLTVTVN---------------

General information: TITO was launched using: RESULT: Template: 5DS1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 310 -4132 -13.33 -46.95 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -13.33 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_5DS1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DS1-query.scw PDB file : Tito_Scwrl_5DS1.pdb: