Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WDLLPSAEQVGKLVP--SAEQIGKWVLSK------EQIDALFFYQCQVTIHYPGAERG------------KLLKYLAEPREP-IPLPKFQ--------GKLYYAISDSGC-KYREYSKGDPWPKG-LKQMDCFFGPRCTAIGKRKIVLQV---------------- 1JTG Chain:B ((1-165)) -AGVMTGAKFTQIQFGMTRQQVLDIAGAENCETGGSFGDSIHCRGHAAGDYYAYATFGFTSAAADAKVDSKSQEKLLAPSAPTLTLAKFNQVTVGMTRAQVLATVGQGSCTTWSEYYPAYPSTAGVTLSLSCFDVDGYSSTGFYRGSAHLWFTDGVLQGKRQWDLV

General information: TITO was launched using: RESULT: Template: 1JTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 470 4698 10.00 39.81 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 10.00 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.179

(partial model without unconserved sides chains): PDB file : Tito_1JTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JTG-query.scw PDB file : Tito_Scwrl_1JTG.pdb: