Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---KKEKDADTDPDTKMCDFVIKKS----------GK--------------KITSGSVWPGATENFMVDGQWPVVSAT----------------QQCRITMS--GLSDIDQYT---------ITLCDKKEDETVSSDGRNTTVISSHGEWC 2CM4 Chain:A ((6-150)) CTGSEPVDAFQAFSEGKEAYVLVRSTDPKARDCLKGEPAGEKQDNTLPVMMTFKQGTDWASTDWTFTLDG--AKVTATLGQLTQNREVVYDSQSHHCHVDKVEKEVPDYEMWMLDAGGLEVEVECCRQKLEELAS----GRNQMYPHLKDC

General information: TITO was launched using: RESULT: Template: 2CM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 320 17470 54.59 191.98 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 54.59 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_2CM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CM4-query.scw PDB file : Tito_Scwrl_2CM4.pdb: