Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---SLLSTISNDKCFVSSYKFTPDGRKVIEASQAAPGDGP---ITFTSTRDNFHMTI--QIDANCAPRNPDDIQKELPSHRGFDFYTKAHNLPGLNPRHN 3TSS Chain:? ((5-93)) NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNT---DGSISLIIFPSPYYSPAFTKGEKVDL-----NTKRIKKSQHTSEGTWIHFQ---ISGVTNTEK

General information: TITO was launched using: RESULT: Template: 3TSS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 277 33743 121.81 416.57 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 121.81 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_3TSS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TSS-query.scw PDB file : Tito_Scwrl_3TSS.pdb: