Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PGNRGSKLSPI--SSSISSPESLS------------DEEFEKFCCVYIVNLGKPDQREWPVLTGKEVWFEHKRKKYDVMAFDSCNLVWGRRGEPKGVTFEFL-ETRTANKDGTC 1O0P Chain:A ((1-104)) -----GHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGK-IFVEFTSVFDCQKAMQGLT---GRKFANRVVVTKYCDPDSYHRRDFW-

General information: TITO was launched using: RESULT: Template: 1O0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 324 7269 22.43 81.67 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 22.43 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_1O0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O0P-query.scw PDB file : Tito_Scwrl_1O0P.pdb: