Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------LCTIDILFRGRRVTFRNIPSGSFQQTEVQGKRCIISVDDSCQPTQVGLDQNYWSVKLREEALGL------------------------------------------------------------------------------------------------------ 1DYR Chain:? ((2-206)) NQQKSLTLIVALTTSYGIGRSNSLPWKLKKEISYFKRVTSFVPTFDSFESMNVVLMGRK-TWESIPLQFRP---LKGRINVVI---TRNESLD-LGNGIHSAKSLDHALELLYRTYGSESSVQINRIFVIGGAQLYKAAMDHPKLDRIMATIIYKDIHCDVFFPLKFRDKEWSSVWKKEKHSDLESWVGTKVPHGKINEDGFDYEFEMWTRDL

General information: TITO was launched using: RESULT: Template: 1DYR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 64 2363 36.92 42.20 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 36.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.703

(partial model without unconserved sides chains): PDB file : Tito_1DYR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DYR-query.scw PDB file : Tito_Scwrl_1DYR.pdb: