Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QCNHHLLYNGRHWGTIRKKAGWAVRFYEEKPGQPKRLVAICKNASPVHCNYLKCTNLAAGFSAGTS-TDVLSSG--TVGSIGNDPQAQRQ------------------- 7TAA Chain:? ((382-476)) -YKNWPIYKDDTTIAMRKGTD----------GS--QIVTILSNKGASGDSYTLSLS-GAGYTAGQQLTEVIGCTTVTVGSDGNVPVPMAGGLPRVLYPTEKLAGSKICS

General information: TITO was launched using: RESULT: Template: 7TAA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 247 -6347 -25.69 -86.94 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -25.69 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_7TAA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-7TAA-query.scw PDB file : Tito_Scwrl_7TAA.pdb: