Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------MLKDKEPGSFIVRDSHSFRGAYGLAMKVATPPPSVLQLNKKAGDLANELVRHFLIECTPKGVRLKGCSN-EPYFGSLTALVCQH----------------------------------------------------------------- 2IZV Chain:A ((17-179)) NLYFQSMLVPDLLQINNNPCYWGVMDKYAAEALLEGKPEGTFLLRDSAQEDYLFSVSFRR------------------YSRSLHARIEQWNHNFSFDAHDPCVFHSPDITGLLEHYKDPSACMFFEPLLSTPLIRTFPFSLQHICRTVICNCTTYDGIDALPIPSSMKLYLKEYHYKSKVR

General information: TITO was launched using: RESULT: Template: 2IZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 157 -5132 -32.69 -78.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -32.69 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_2IZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IZV-query.scw PDB file : Tito_Scwrl_2IZV.pdb: