Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDV-TLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN 4K3L Chain:B ((1-366)) -MKFTVEREHLLKPLQQVSG-------LPILGNLLLQVADGTLSLTGTDLEMEMVARVALV------QPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGE-RMLVRSGRSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLFETE-GEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQIGSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNEKFRG-VRLYVSENQLKITANNPEQEEAEEILDV-TYSGAEMEIGFNVSYVLDVLNALKCENVRMMLTDSVSSVQIEDA-ASQSAAYVVMPMRL-

General information: TITO was launched using: RESULT: Template: 4K3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1940 -36443 -18.79 -101.80 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -18.79 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_4K3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K3L-query.scw PDB file : Tito_Scwrl_4K3L.pdb: