Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETESILGLGG--KVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA---DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEPFILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVP------SASWIAAGDYHHHLAVNEWRGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDA--PIKWM-------TSSKLEITDSDGIVTRIRLAR 4Z6M Chain:B ((9-271)) -VAPAPDILRCAYAELVVTDLAKSRNFYVDVLGLHVSYEDENQIYLRSFEEFIHHNLVLTK-G-----PVAALKAMAFRVRTPEDVDKAEAYYQELGCRTERRKDGFVKGIGDALRVEDPLGFPYEFFFETTHVER-L-HM--------RY------------DLYSAGELVRLDHFNQVTPDVPRGRKYLED-LGFRVTEDIQDDEGTTYAAWMHRKGTVQDTALTG----------GNGPRLHHVAFSTHEKHNIIQICDKMGALRISDRIERGPGRHGVSNAFYLYILDPDNHRIEIYTQ-

General information: TITO was launched using: RESULT: Template: 4Z6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1265 -15781 -12.47 -64.94 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -12.47 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_4Z6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z6M-query.scw PDB file : Tito_Scwrl_4Z6M.pdb: