Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINE-EQGQFGFDRVKELVDT--YKVERFVVGLPKNMNNTSG-----P-R-VEASQAYGAKLEEFFGLPVDYQDERLTTVAAERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF 2AA4 Chain:A ((1-113)) MTTLAIDIGGTKLAAALIGADGQIR-DRRELPTPASQTPEALRDALSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIAINDAQAAAWAE-----------------------------------

General information: TITO was launched using: RESULT: Template: 2AA4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 467 -18676 -39.99 -181.32 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -39.99 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_2AA4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AA4-query.scw PDB file : Tito_Scwrl_2AA4.pdb: