Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANGRGAYIKL-DNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHK----VKRRELGLE---- 5AZX Chain:A ((1-100)) GSISFHLPVNSRKCLREEIHKDLLVTGAYEIT-----DQSGGAGGLRTHLKITDSAGHILYAKEDATKGKFAFTTED--YDMFEVCFESKGTGRIPDQLVILDMKHG

General information: TITO was launched using: RESULT: Template: 5AZX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 362 32563 89.95 361.81 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 89.95 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.088

(partial model without unconserved sides chains): PDB file : Tito_5AZX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AZX-query.scw PDB file : Tito_Scwrl_5AZX.pdb: