Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISLQDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSRLLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIKNG-QDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQN-----DRLYITDFFDFSIYVDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNLQNYIEPTRNRAEVILHKSKNHEIDEIYLKK 4NE2 Chain:B ((32-334)) ---------QFNRHQWAALR-------TEDEITRLKGINEDLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKSTTARVLQALLSRWPEHRHVELITTDGFLHPNSVLKERGLMKKKGFPQSYDMHRLVKFVSDLKSGVPQATAPVYSHLIYDVIPDGDKTVAQPDILILEGLNVLQSGMDYPHDPHHVFVSDFVDFSIYVDAPEELLKSWYINRFLKFREGAFTDPDSYFHNYAKLSKEEAVDIATSLWNEINLMNLKENILPTRERASLIMTKSANHSVNQVRLRK

General information: TITO was launched using: RESULT: Template: 4NE2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1347 -33726 -25.04 -116.29 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -25.04 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_4NE2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NE2-query.scw PDB file : Tito_Scwrl_4NE2.pdb: