Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMAVYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVNKHSTGGVGD-KVTLILVPLVASFGVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQL--VKADKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELAQTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEIL-QGQGRQDITHFICELAQIMLGLANVNKTV----EEVRQHLENGQALAKFEEMVQAQGGDLED-LYRPV-NVAHVVEIPAQETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYTNEKIP-PQLVTDFQKCVKISDEVKKIREIVEIIS 4GA5 Chain:A ((86-492)) -ESVRYIKKKMHGEKLRKVEIEAIVRDIVDRKLRDIEISSFVTALEINGLDMDEIAALTIAMAETGDMLDIDR--KPIMDVHSIGGVPGNKTNILVVPIVAAAGLTIPKTSSRAITSAAGTADVVEVF-A-DVSFSLDEIKRIVEKVGACLVWG-GALNLAPADDITIKAERAL-SIDPTGLMLASIMSKKYAMGSQYVLIDIPTGKGVKVETVEEARSLARDFIELGKRLGQYVEVAITYGGQPIGHTVGPALEAREALSALMTGKGPGSLIEKATGLAGILLEMGGVA-PAGTGKKMAKEILESGKAWEKMKEIIEAQGGDPNIKPE-EIPIGDKTYTFTAATSGYVTAIDNRAITAIARAAGAPED-------KGAGIELYVKVGEKVKEGDPLFTIHAEHEARLDQAIVLARRTEPIRI-------------

General information: TITO was launched using: RESULT: Template: 4GA5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2416 -48275 -19.98 -121.91 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -19.98 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_4GA5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GA5-query.scw PDB file : Tito_Scwrl_4GA5.pdb: