Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQKYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAY-KQPITRIEIDAIRG------VN---SSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHLEELPVIDELEIQAQESQLFGERIEEDENQ 2CO5 Chain:B ((5-81)) ------------------------------------------------------------------------------------KYMRINYYIILKVLVINGSRLEKKRLRSEILKRFDIDISDGVLYPLIDSLIDDKILREEE----APDGKVLFLTEKGMKEF----------------------------------

General information: TITO was launched using: RESULT: Template: 2CO5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 201 -5675 -28.23 -84.69 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -28.23 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_2CO5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CO5-query.scw PDB file : Tito_Scwrl_2CO5.pdb: