Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKRTWRNSFVTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKE-LGAGLVVMEMVSDKGIQYNNEKTL------HMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVL--DIPLTVKMRTGWADPSLAVENALAAEAAGVSALAMHGRTREQMYTG-HADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA 4YCO Chain:C ((6-318)) -------------------------SMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVE-LGSWGVDLNCGAPS----GSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDIRQRL-NIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYNEPRM-----PW-PEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDI

General information: TITO was launched using: RESULT: Template: 4YCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1693 22704 13.41 75.93 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 13.41 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_4YCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YCO-query.scw PDB file : Tito_Scwrl_4YCO.pdb: