Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIQLYLLKLELFWFELHQHYHFSEPQKILNELIAAYSAAYSEKQRAYHTVQHLYECLSLIETVQSE--------LNDPYAVALALWFHDVVYEPQA-------------------LDNELKSAELFEQLMAQ-DL------QL----DTMQKIKRWILATQKHAPT----------------------DETDLQFLLDIDLAILAATPERFMQYEQQIQQEYSWADPEVYSIKRKEVLMNFYQSEPLYQTAYFQKNFELNAKQNLKKILE 5U00 Chain:C ((66-256)) ---------------------------RFCLMVKKG----YR--DPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFF-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5U00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 475 -9876 -20.79 -75.39 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.57 3D Compatibility (PKB) : -20.79 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_5U00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U00-query.scw PDB file : Tito_Scwrl_5U00.pdb: