Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDASPSVQMIAALATFSVPFIFRPLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLI---PSYETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGAATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQH----SEQQAQ------------------------KSKPQKFSFKEIFVKHK--RSLLVCIGLVISTNV-TYYMLLTYLPSYFSHNLGYSEAHGALIIIAVMVGMLFVQPVIGYLSDKFGRRPFIFIGSFSLIFLSYPAFVL-L-N-S---GVNYQIFIGLLILALSLNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTPTLTATLVETTHNLMVPAYYLMICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQELQQKRQKLADKHPDIA 5EQH Chain:A ((70-430)) -------------------------------------------------------------------------AIFSVGGMIGSFSVGLFVNRFGRRNSMLMMNLLAFVSAVLMGFSKLGKSF--------EMLILGRFIIGVYCGLTTGFVPMYVGEVSPTALRGALGTLHQLGIVVGILIAQVFGLDSIM------GNKDLWPLLLSIIFIPALLQCIVLPFCPESPRFLLINRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQLSQQLSGINAVFYYSTSIFE-KAGV--QQPVYATIGSGIVNTAFTVVSLFVVERAGRRTLHLIGLAGMAGCAILMTIALALLEQLPWMSYLSIVAIFGFVAFFEVGPGPIPWFIVAELFSQGPRPAAIAVAGFSNWTSNFIVGMCFQYVEQLCG------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EQH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1191 -136087 -114.26 -423.95 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -114.26 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_5EQH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQH-query.scw PDB file : Tito_Scwrl_5EQH.pdb: