Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTTYIGLGSNLGDSRQILSEAITKLKSLGEV------KVSKLYQSPPMGPQDQPNYLNAVAQLKTELLPLELLDQLQRFEQEAGRVR-LRHWGERTLDLDLLIYGNEKIQNERLTVPHVGILQRDFVVIPLLDLDADLHINDQPLKNLELIQQPTLTVLADESWA 3ILO Chain:A ((1-139)) --TVAYIAIGSNLASP---LEQVNAALKALGDIPESHILTVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLAIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPD-----------------------

General information: TITO was launched using: RESULT: Template: 3ILO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 660 13976 21.18 105.88 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 21.18 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_3ILO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ILO-query.scw PDB file : Tito_Scwrl_3ILO.pdb: