Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRDTVFLKVESRLGYDFSGDIKIGGKYTSLIEHAGLVFISGQIPRVGDTVQICGKVGLDVDLSDAQLAASISTMRALAILKQHYGTLDVIGKVLQMNVFVHSTSSFIQQSEVADGASEILYEILGLDTGRHTRTSVSVYQLPKNASVEINFIFALK 3QUW Chain:A ((25-144)) ----------------STKLAPPAAASYSQAMKANNFVYVSGQIPYTPDNKPVQG----SI-SEK----AEQVFQNVKNILAESNSSLD---NIVKVNVFLADMKNFAEFN-------SVYAKHFH-T-HKPARSCVGVASLPLNVDLEMEVIAVEK

General information: TITO was launched using: RESULT: Template: 3QUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 559 -50695 -90.69 -422.45 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -90.69 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_3QUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QUW-query.scw PDB file : Tito_Scwrl_3QUW.pdb: