Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQLLQQNQIADENCHVGVAPVSIALTTVQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGLCQLTPAGSNIA----ILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 1SUX Chain:A ((7-249)) -------PIAAANWKCNGSESLLVPLIETLNA-----ATFDHDVQCVVAPTFLHIPMTKARLTNPKFQI--AAQNA--ITRSGAFTGEVSLQILKDYGISWVVLGHSERRLYYGETNEIVAEKVAQACAAGFHVIVCVGETNEEREAGRTAAVVLTQLAAVAQKLSKEAW-SRVVIAYEPVWAIGTGKVATPQQAQEVHELLRRWV--RSKLGTDIAAQLRILYGGSVTAKNARTLYQMRDINGFLVGGASLK-PEFVEIIEA-----

General information: TITO was launched using: RESULT: Template: 1SUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1280 -129046 -100.82 -539.94 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -100.82 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_1SUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SUX-query.scw PDB file : Tito_Scwrl_1SUX.pdb: