Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLA----NPQTTHAAIAVP--TSTVIHKTIEMDA----DMSDDEREVQIR----------VDAEQYIPFP----LDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLELADLNPKLADVESYAVERTFSVFADSLPIGAN-TIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEEASRAKKDRSLP-------------------------DDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFLQMGFSPQVDVQKIENDASSLMVACGLALRSFD 3WT0 Chain:C ((25-388)) VSIDIGSSSVKTIVGEKFHNGINVIGTGQTY--TSGIKNGLIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEINGSHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARH--SLKVEAGVIAIQKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATE------KELGACVIDIGEDVTQVAFYERGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQVDSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLT---KVNGGFIVTGGSANLLGVKELLSDMVSEKVRIHTPSQM------------GIRKPEFSSAISTISSSI-

General information: TITO was launched using: RESULT: Template: 3WT0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1570 3287 2.09 10.47 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 2.09 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_3WT0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WT0-query.scw PDB file : Tito_Scwrl_3WT0.pdb: