Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAFKDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAP-DLPAKNFTAMLRLDHNRALTQVAQKAGVVVADIEKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHMRDWALGT-NGKWVTMGVPSD-GSYGIPEGVMFGFPVTTENGEYKIVQGLEIDEFSRERINFTLNELEEERAAIADMVK 4I1I Chain:A ((25-341)) ----VKVAVTGAAGQIGYALVPLIARGALLGPTTPVELRLLDI--EPALKALAGVEAELEDCAFPLLDKVVVTADPRVAFDGVAIAIMCGAFPRKAGMERKDLLEMNARIFKEQGEAIAAVAASDCRVVVVGNPANTNALILLKSAQGKLNPRHVTAMTRLDHNRALSLLARKAGVPVSQVRNVIIWGNHSSTQVPDTDSAVIGTTPAREAIKD-------FVQVVRGRGAEIIQLRGLSSAMSAAKAAVDHVHDWIHGTPEGVYVSMGVYSDENPYGVPSGLIFSFPCTCHAGEWTVVSGKLNGDLGKQRLASTIAELQEERA-------

General information: TITO was launched using: RESULT: Template: 4I1I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1763 -124004 -70.34 -402.61 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -70.34 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4I1I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I1I-query.scw PDB file : Tito_Scwrl_4I1I.pdb: