Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTATALTLACSLLSVMSISQAQAADNIDVSFQTILQQERNWAGLQSKSLKVGDITWSYSEGGSSTKPTLLLIHGLAGSRDNWNRVAHYLTTNYHVIIPDLPGSGETIVSHDFDYSVPNLAEKLRRFVEAANLKGPIHIAGHSLGGSIALLYAGQYPFETKSLFLVDSGGIFRSANT--IYLKDLTYLKQLLVSKK--G---DFNYLLKQTMFNP---PFIPREFLQAQEKLMIN-QAPQTQKLVD------QLIALNKVYTPDSFAVLTKTIDAPTLILWGKQDKIINVEVANELKRLLKNAQPPVILENVGHMPILEAEQLVIQQYVPFLLKVETNQSSKTTTP 3G9X Chain:A ((11-295)) ----------------------------------------------DPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYLWRNIIPHVAPSHRCIAPDLIGMGKSDKP-DLDYFFDDHVRYLDAFIEALG-LEEVVLVIHDWGSALGFHWAKRNPERVKGIACMEFIRPFPTWDEWPEFARETF--QAFRTADVGRELIIDQNAFIEGALPKCVVRPLTEVEMDHYREPFLKPVDREPLWRFPNELPIAGEPANIVALV--EAYMNWLHQSPVPKLLFWGTPGVLIPPAEAARLAESLPN-CKTVDIGPGLHYLQEDNPDLIGSEIARWLPALHH---------

General information: TITO was launched using: RESULT: Template: 3G9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1446 -98846 -68.36 -368.83 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -68.36 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_3G9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G9X-query.scw PDB file : Tito_Scwrl_3G9X.pdb: