Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAFNVERITYVHHWNDTLFSFKTTRDASLRFKNGQFVMIGLEVNGKPLMRAYSIASANYEEELEFFSIKVQDGPLTSILQKVQVGDEILVS-KKPTGTLVHDDLLPGKNLYLLSSGTGLAPFLSIIRDPETYERFEKVIVVHGTRYISELAYQDLILNELPNNEFFEELGIKDKLVYYPTVTREPFHTQGRVTTAIETGA-LFEKIGLPRFNRETDRAMLCGSPAFLKDVAALLDQHGLVESPRMGEMGDYVIERAFVEK 4FK8 Chain:B ((18-271)) --KFDTATVLSVHHWTDTLFSFTCTRDQALRFNNGEFTMVGLEVDGKPLTRAYSIVSPNYEEHLEFFSIKVQNGPLTSRLQHLKVGDPVLIGKKP-TGTLVADNLLPGKTLWMLSTGTGLAPFMSIIRDPDIYERFDKVVLTHTCRLKGELAYMDYIK-HDLPGHEYLGDVIREKLVYYPTV---------RITDLIASGKLFTDLDMP-PFSPEQDRVMLCGSTAMLKDTTELLKKAGLVEGKNS-APGHYVIERAFVD-

General information: TITO was launched using: RESULT: Template: 4FK8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1346 -78061 -57.99 -321.24 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -57.99 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_4FK8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FK8-query.scw PDB file : Tito_Scwrl_4FK8.pdb: