Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEIDAHPR-EVGSGHVRVEIKAFSVNPYDVALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVT----TVHVGDRVAVHAVGGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASS-KNEEKVKKLGASAFAAYDKTN--PGLQFA-----DQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDL--DLRQKK---EG---FYE-S-FVPR-KEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEG-HPPAGKIILSFEK 4RVS Chain:A ((2-328)) MHAIEVTETGGP-GVLRHVDQ-PQPQPGHGELLIKAEAIGVNFIDTYFRSGQYP----RELPFVIGSEVCGTVEAVGPGVTAADTAISVGDRVVSASANGAYAEFCTAPASLTAKVPDDVTSEVAASALLKGLTAHYLLKSVYPVKRGDTVLVHAGAGGVGLILTQWATHLGVRVITTVSTAEKAKLSKDAGADVVLDYPEDAWQFAGRVRELTGGTGVQAVYDGVGATT-FDASLASLAVRGTLALFGAASGPVPPVDPQRLNAAGSVYLTRPSLFHFTRTGEEFSWRAAELFDAIGNEAITVAVGGRY--PLADALRAHQDLEARK-TVGSVVLL---

General information: TITO was launched using: RESULT: Template: 4RVS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1744 40432 23.18 133.88 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 23.18 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_4RVS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RVS-query.scw PDB file : Tito_Scwrl_4RVS.pdb: