Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRGRTKMNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISSAV-------SMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI 2R4Q Chain:A ((170-237)) --------AKILAVTACPTG--HTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQEIEDAPAIIVAADKQVE--------------------------------------

General information: TITO was launched using: RESULT: Template: 2R4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 196 -4278 -21.83 -72.51 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -21.83 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_2R4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R4Q-query.scw PDB file : Tito_Scwrl_2R4Q.pdb: