Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRRKPMKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLLESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEEGNALLTKK--TLES-QVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKSGFYFEWKNLENYFLE-----TRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEK-GKAATVEKKIDGW--EQNTEKLRIDFIFVPEGMQVKKYQRIFDG---IDSPIISDHYGVEIELDVNE 4FVA Chain:C ((9-255)) --LKGFEVSVMSWNIDGLDGRSLLTRMKAVAHIVKNVNPDILFLQEVVDRDLAPID----------------------------KL---QSLYKIYYSN-----KGCQYYTAILVSKMFDVEKHDVIHFQN-----SGMYRTLQILEGSIGGLKVFLLNTHLESTREHRPQRCAQFGFCMDKVREIIAQNPGALVFFGGDLNLRDEEVS--RVP-D--GVKDAWEAAGSDNKTKFTWDTFKNDNKQGFHGAKMRFDRLYWSGPLDKVKFTLEGRQRIRSCLCFPSDHWAINATFF---

General information: TITO was launched using: RESULT: Template: 4FVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1171 17463 14.91 74.95 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 14.91 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_4FVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FVA-query.scw PDB file : Tito_Scwrl_4FVA.pdb: