Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKENFKRSVSRMNAFRSFQVIKEP-VFHTAISDLPFTELAENEVLVKIAYSDVNYKDALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQYQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGM-TSDKKVVVTGASGGVGSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDYVIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPIDQRLSLWQQMADLQIA--DEL-VVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK 1Y9E Chain:F ((2-330)) ------------STLFQALQAEKNADDVSVHVKTISTEDLPKDGVLIKVAYSGINYKDGLAGKAGGNIVREYPLILGIDAAGTVVSSNDPRFAEGDEVIATSYELGVSRDGGLSEYASVPGDWLVPLPQNLSLKEAMVYGTAGFTAALSVHRLEQNGLSPEKGSVLVTGATGGVGGIAVSMLNKRGYDVVASTGNREAA-DYLKQLGASEVISREDVYDGTLKALSKQQWQGAVDPVGGKQLASLLSKIQYGGSVAVSGLTGGGEVPATVYPFILRGVSLLGIDSVYCPMDVRAAVWERMSSDLKPDQLLTIVDREVSLEETPGALKDILQNRIQGRVIVKL----

General information: TITO was launched using: RESULT: Template: 1Y9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 2008 -38268 -19.06 -118.11 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain F : 0.84 3D Compatibility (PKB) : -19.06 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_1Y9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y9E-query.scw PDB file : Tito_Scwrl_1Y9E.pdb: