Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGKTILVTGGTGSIGSEIVRQLLKY-EPKAIRIYSRGEEKQFYMQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVRFGNVMGSSGSVIPLFSKQIKNN-KDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATDE------LYK--DYEKIKITAYSSDATE-LMSGQEFIRIAKENNVVTW 2GN4 Chain:A ((19-344)) -LDNQTILITGGTGSFGKCFVRKVLDTTNAKKIIVYSRDELKQSEMAMEFN-DPRMRFFIGDVRDLERLNYALEGVDICIHAAALKHVPIAEYNPLECIKTNIMGASNVINACLKNAISQVIALSTDKAANPINLYGATKLCSDKLFVSANNFKGSSQTQFSVVRYGNVVGSRGSVVPFFKKLVQNKASEIPITDIRMTRFWITLDEGVSFVLKSLKRMHGGEIFVPKIPSMKMTDLAKALAPNTP----------TKIIGIRPGEKLHEVMIPKDESHLALEFEDFFIIQPTISFQTPKDYTLTKLHEKGQKVAPDFEYSSHNNNQWLEPDDLLKLL--------

General information: TITO was launched using: RESULT: Template: 2GN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1759 -23617 -13.43 -74.97 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -13.43 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_2GN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GN4-query.scw PDB file : Tito_Scwrl_2GN4.pdb: