Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSIAEYFHYYGEKSFRKVESDILRKLSN--EDKIIATGGGIVQSAENRRFLKTQPIVLYLEAEADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIIDTIIERIKSI 3N2E Chain:B ((1-145)) MQHLVLIGFMGSGKSSLAQELGLALKLEVLDTDMIISERVGLSVREIFEELGEDNFRMFEKNLIDELKTLKTPHVISTGGGIVMH-ENLKGLGT---TFYLKMDFETLIKRLNQKERAKRPLLNNLT--QAKELFEKRQALYEKNASFIID--------------------

General information: TITO was launched using: RESULT: Template: 3N2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 577 -30215 -52.36 -211.29 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -52.36 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_3N2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N2E-query.scw PDB file : Tito_Scwrl_3N2E.pdb: