Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQEMMEIVQQKQLAPKIFEMTLKGDL-VKEMQQPGQFLHILVPRAD-----------------------------LLLRRPISLNHIDHETNTCRIIYRVE-----------------GEGTRVFSTLSAGDHLDVMGPLGNGFDLSNLHTGDEVFIIGGGIGVPPLYELSKQLVEK---GIKPTHFLGFATHEVVYYEKEFNRL-G-----ETRIATD-DG----TYGMHGNVGNLL-LGTK-----TEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRMACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII 2R6H Chain:C ((8-277)) -KEWECEVLSNKNVSTFIKEFVVKLPEGETMNFKSGSYAQIKIPKYNIRYADYDIQDRFRGDWDKMDAWSLTCKNEEETVRAYSMANYPAEGNIITLNVRIATPPFDRAANKWKAGIKPGISSSYIFSLKPGDKVMMSGPYGD-FHIQDT--DAEMLYIGGGAGMAPLRAQILHLFRTLKTGRKVSYWYGARSKNEIFYEEDFREIEREFPNFKFHIALSDPQPEDNWTGYVGFIHQVIYDNYLKDHDAPEDIEYYMCGPGPMANAVKGMLENL---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2R6H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 992 684 0.69 3.37 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 0.69 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_2R6H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R6H-query.scw PDB file : Tito_Scwrl_2R6H.pdb: