Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIVPEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIEAGT-GIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGAGGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGEEVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFLRSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA 5F7Q Chain:E ((85-392)) -YGFVVSVKVEEEQLLFALTDLNAEIIENTSIPFSSEKKPEEAIELIAKNVKKMCGN--RDMNHLLGVGIAISGLVNRKKGTVIRSTMLGWENVALEAMLHAH-FPDIPVYVDKNINCYTLAELWLGEGKQSNNFATVSVGAGLGLSVVINRQIYYGAQGGAGEFGHTTIQPGGYKCHCGQKGCLEMYASEFYFRNRGEELKEAYPTS--------ELNDFHFDKVAKSARAGDEMATELMGKMGEYLGYGIRNIINTFNPEKVIIVGEGLHHRDLFLTKIDEIASQNFFSGAGFETEITTTSLEDPAWLQGAALLVIHQ-

General information: TITO was launched using: RESULT: Template: 5F7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1750 -78920 -45.10 -257.07 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.74 3D Compatibility (PKB) : -45.10 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_5F7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F7Q-query.scw PDB file : Tito_Scwrl_5F7Q.pdb: