Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTVKKQNDGTTFLLTG--DIPAMWLRDSTAQVRPYLVIAKEDEEL-ADMISGLVKKQFF-YINIDPYANAFNEE--ANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKT----DQFNQSFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWS-----------------------GFRPSD-----------------------------------------DTCRYGYLVPSNMFAVVVLGYVEEIFQTILD-DKEIVQQTQKLKKEIQEGIQNHGRTVNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSK----ENPY--FYEGKYAKGIGSSHTPEKYVWPIALAMEGM-TTSDKSEKERILNLLVATDAGTHLMHEGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK 4WVA Chain:B ((3-419)) -----------------------------PLRTKAV-EVLQRNSRGA-----FTVPAHGLYPYQWLWDSAFIALGWTQVD--WERAWQELLCLFDYGQGPDGMLPHIVFHEQSRDYFPGPDVWGR-----QP----ATSGITQPPVVATVVRYLYEKDPDRDRARERARY-LFPKLLAFHRWLYHARDPY---------------------------RTGLVVIVHPWESGMDNSPAWDKPLSRVPVENLPPYERRDVKHVNPEERPRKEDYDRYLSLLYLFRRLEYDPREIYRQSPFKVVDVGFNAILQRANRDLYALAVLLQEDP-YEIEEWIVRGEV----GLEALWDREA-GFYFSW--DLVAGEP--IAVK-TSAGFLPLFAGTPHQGR--ASLLAQEAERWGEKARYLLPSVDPTSPFFEPGRYWRGPVWINVNWMVAEGFRDYGFAALAARLKADALALME-REGFREYYDPLTGQGRGGEGFSWSAALALFWT----------

General information: TITO was launched using: RESULT: Template: 4WVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1693 -32802 -19.37 -99.10 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -19.37 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_4WVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WVA-query.scw PDB file : Tito_Scwrl_4WVA.pdb: